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Addgene

mCNMG
(Plasmid #1255)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1255 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 5276
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUP35 NM-domain
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1664
  • Mutation
    Fusion between the NM-domain of SUP35 and sGFP
  • GenBank ID
    AY553990
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Tag / Fusion Protein
    • sGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Sac1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCNMG was a gift from Susan Lindquist (Addgene plasmid # 1255 ; http://n2t.net/addgene:1255 ; RRID:Addgene_1255)

  • For your References section:

    Molecular population genetics and evolution of a prion-like protein in Saccharomyces cerevisiae. Jensen MA, True HL, Chernoff YO, Lindquist S. Genetics 2001 Oct;159(2):527-35. PubMed 11606530
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