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Addgene

pSuper.mBeta826
(Plasmid #12549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER-Retro
  • Backbone manufacturer
    Oligoengine
  • Backbone size w/o insert (bp) 4488
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA to mouse polymerase beta
  • Species
    shRNA oligonucleotide
  • Insert Size (bp)
    65

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ccctttatccagccctcactc
  • 3′ sequencing primer tttgcatgtcgctatgtgttc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

shRNA oligo sequence - 5'-gatcagtactactgtggtg

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuper.mBeta826 was a gift from Robert Sobol (Addgene plasmid # 12549 ; http://n2t.net/addgene:12549 ; RRID:Addgene_12549)

  • For your References section:

    The role of base excision repair in the sensitivity and resistance to temozolomide-mediated cell death. Trivedi RN, Almeida KH, Fornsaglio JL, Schamus S, Sobol RW. Cancer Res. 2005 Jul 15. 65(14):6394-400. 10.1158/0008-5472.CAN-05-0715 PubMed 16024643
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