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Addgene

pLPC hRap1-FL
(Plasmid #12542)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12542 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Repressor Activator Protein 1
  • Alt name
    Rap1
  • Alt name
    1085
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • GenBank ID
    AF262988
  • Entrez Gene
    TERF2IP (a.k.a. DRIP5, RAP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAGGCGTGTACGGTGGGA
  • 3′ sequencing primer CTTGCCAAACCTACAGGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC hRap1-FL was a gift from Titia de Lange (Addgene plasmid # 12542 ; http://n2t.net/addgene:12542 ; RRID:Addgene_12542)

  • For your References section:

    Rap1 affects the length and heterogeneity of human telomeres. Li B, de Lange T. Mol Biol Cell. 2003 Dec . 14(12):5060-8. 10.1091/mbc.e03-06-0403 PubMed 14565979
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