pFS181
(Plasmid #12536)

• Depositing Lab: Nick Rhind
• Publication: Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Backbone

• Vector backbone: pJK148
• Backbone manufacturer: Keeney and Boeke
• Backbone size w/o insert (bp): 5343
• Vector type: Yeast Expression
• Selectable markers: leu1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human equilibrating nucleoside transporter 1
• Alt name: hENT1
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• GenBank ID: AAC51103
• Entrez Gene: SLC29A1 (a.k.a. AUG, ENT1, hENT1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (destroyed during cloning)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: IMAGE Consortium
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry