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Addgene

pFS181
(Plasmid #12536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12536 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJK148
  • Backbone manufacturer
    Keeney and Boeke
  • Backbone size w/o insert (bp) 5343
  • Vector type
    Yeast Expression
  • Selectable markers
    leu1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human equilibrating nucleoside transporter 1
  • Alt name
    hENT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    AAC51103
  • Entrez Gene
    SLC29A1 (a.k.a. AUG, ENT1, hENT1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IMAGE Consortium
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFS181 was a gift from Nick Rhind (Addgene plasmid # 12536 ; http://n2t.net/addgene:12536 ; RRID:Addgene_12536)

  • For your References section:

    In vivo labeling of fission yeast DNA with thymidine and thymidine analogs. Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N. Methods. 2004 Jul . 33(3):213-9. 10.1016/j.ymeth.2003.11.016 PubMed 15157888
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