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Addgene

Bov.hsc70.RSET.a1-554.wt(NarI)
(Plasmid #12533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET_A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bovine 70 kDa heat shock cognate protein fragment amino acids 1-554
  • Alt name
    Hsc70 60 kDa fragment
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1662
  • Mutation
    deleted carboxy-terminal residues 555-650 of bovine Hsc70 protein

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bov.hsc70.RSET.a1-554.wt(NarI) was a gift from David McKay (Addgene plasmid # 12533 ; http://n2t.net/addgene:12533 ; RRID:Addgene_12533)

  • For your References section:

    Destabilization of peptide binding and interdomain communication by an E543K mutation in the bovine 70-kDa heat shock cognate protein, a molecular chaperone. Ha JH, Hellman U, Johnson ER, Li L, McKay DB, Sousa MC, Takeda S, Wernstedt C, Wilbanks SM. J Biol Chem. 1997 Oct 31. 272(44):27796-803. 10.1074/jbc.272.44.27796 PubMed 9346924
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