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pAcGP67A-pIgR D1-H6
(Plasmid #12531)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12531 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGP67A
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 9761
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human polymeric immunoglobulin receptor
  • Alt name
    human pIgR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    350
  • Mutation
    Lys 1 - Val 109
  • GenBank ID
    P01833
  • Entrez Gene
    PIGR
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII/BamHI (destroyed during cloning)
  • 5′ sequencing primer 5' AAT TTAA AAT gAT AAC CAT CTC gC 3'
  • 3′ sequencing primer 5' gAA gCg ggT CCA AgT TTC CCT g 3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Roland Strong

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation numbering does not include the signal peptide (aa1-18 of P01833).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGP67A-pIgR D1-H6 was a gift from Pamela Bjorkman (Addgene plasmid # 12531 ; http://n2t.net/addgene:12531 ; RRID:Addgene_12531)

  • For your References section:

    Crystal structure of a polymeric immunoglobulin binding fragment of the human polymeric immunoglobulin receptor. Hamburger AE, West AP, Bjorkman PJ. Structure. 2004 Nov . 12(11):1925-35. 10.1016/j.str.2004.09.006 PubMed 15530357
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