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pET20b-pIgR D1-H6
(Plasmid #12530)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12530 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET20b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3716
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human polymeric immunoglobulin receptor
  • Alt name
    human pIgR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    350
  • Mutation
    Lys 1-Val 109
  • GenBank ID
    P01833
  • Entrez Gene
    PIGR
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Roland Strong

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET20b-pIgR D1-H6 was a gift from Pamela Bjorkman (Addgene plasmid # 12530 ; http://n2t.net/addgene:12530 ; RRID:Addgene_12530)

  • For your References section:

    Crystal structure of a polymeric immunoglobulin binding fragment of the human polymeric immunoglobulin receptor. Hamburger AE, West AP, Bjorkman PJ. Structure. 2004 Nov . 12(11):1925-35. 10.1016/j.str.2004.09.006 PubMed 15530357
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