pBS MCK promoter
(Plasmid #12528)

• Depositing Lab: Ronald Kahn
• Publication: Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

Backbone

• Vector backbone: pBluescriptKS
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCK promoter
• Alt name: muscle creatine kinase promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 6500
• Entrez Gene: Ckm (a.k.a. CPK-M, Ckmm, M-CK, MCK)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • MCK promoter info
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: See PMID: 3785216 for more information on this promoter
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A 6.5 kb XhoI fragment containing 3 kb of the MCK promoter and enhancer 1, untranslated exon 1, 3 kb of intron 1 including the enhancer region E2, and the first 16 bp of exon 2 was subcloned into the XhoI site of pBluescriptKS to result in pBSMCK. A PolyA sequence was added downstream (see author's map, MCK-polyA-2). Please note that the restriction sites shown between the MCK promoter and polyA region in the author's map are NOT unique sites.