CSII-prEF1a-mCherry-3xNLS
(Plasmid #125262)

• Purpose: Fluorescent reporter for cell size
• Depositing Lab: Jan Skotheim
• Publication: Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2995. doi: 10.1091/mbc.E19-03-0171. Epub 2019 Oct 10.

Backbone

• Vector backbone: CSII
• Backbone size w/o insert (bp): 9104
• Total vector size (bp): 9935
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry-3xNLS
• Species: H. sapiens (human)
• Insert Size (bp): 831
• Promoter: EF1a
• Tag / Fusion Protein:
  • 3xNLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: tgaagttaggccagcttgg
• 3′ sequencing primer: cattaaagcagcgtatcc

Resource Information

• Supplemental Documents:
  • CSII_EF1_mCherry-3xNLS.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/587162v1 for bioRxiv preprint.