pBabe puro HA PIK3CB
(Plasmid #12526)

• Depositing Lab: Jean Zhao
• Publication: Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8.

Backbone

• Vector backbone: pBabe puro
• Backbone manufacturer: Available at Addgene (plasmid #1764)
• Backbone size w/o insert (bp): 5150
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PIK3CB
• Alt name: phosphoinositide 3-kinase
• Alt name: PI3K
• Alt name: p110 beta
• Species: H. sapiens (human)
• Insert Size (bp): 3240
• Entrez Gene: PIK3CB (a.k.a. P110BETA, PI3K, PI3KBETA, PIK3C1)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HMS clone ID number: HsCD00025922. To construct a hemagglutinin (HA)-tagged allele of p110 beta, a cDNA clone of human PIK3CB (obtained from the FLEXGene Repository of the Harvard Institute of Proteomics) was subcloned into pBabepuro by PCR with primers containing HA-epitope coding sequence with subsequent restriction and ligation.