FD1
(Bacterial strain
#125258)
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PurposeDerivative of MG1655 used for the screening of clones able to survive the bad seed toxicity of dCas9, while still efficiently silencing the expression of mcherry
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 125258 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backbonenone
Growth in Bacteria
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Bacterial Resistance(s)None
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Growth Temperature37°C
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Growth Strain(s)This is a strain.
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Growth instructionsGrows on LB medium
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesingle chromosomal copy of yhhX target sequence upstream from mcherry reporter gene under control of a constitutive promoter
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genotype:
The expression of mcherry can be visualized or measured with a spectrophotometer.
The yhhX target sequence followed by a mcherry reporter gene carried by an integrative vector based on pOSIP-KL was integrated at the lambda attB site in the chromosome of E. coli MG1655 and the backbone was flipped out using the pE-FLP (AmpR, #45978, Addgene) plasmid.
This strain can be used to optimize dCas9 expression levels
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FD1 was a gift from David Bikard (Addgene plasmid # 125258) -
For your References section:
Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels. Depardieu F, Bikard D. Methods. 2019 Aug 1. pii: S1046-2023(18)30497-3. doi: 10.1016/j.ymeth.2019.07.024. 10.1016/j.ymeth.2019.07.024 PubMed 31377338