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Addgene

pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid
pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE with the
following enzymes: NheI-HF, SnaBI.

Expected Results: 7346 + 1766 + 949 bp

We observed the expected results.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Ladder and a digestion of the plasmid pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE with the following enzyme: NotI-HF

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE with the following enzyme(s): KpnI-HF.

Expected Bands:
KpnI-HF: 10kb

Results:
We observed the expected band for this digest.