Skip to main content
Addgene

pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE
(Plasmid #125256)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLEX_307
  • Backbone manufacturer
    Addgene #41392 (provided by David Root)
  • Backbone size w/o insert (bp) 8423
  • Total vector size (bp) 10061
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hChR2(H134R)
  • Alt name
    Channelrhodopsin-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1638
  • Promoter EF1a-forward
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE was a gift from Roger D. Kamm (Addgene plasmid # 125256 ; http://n2t.net/addgene:125256 ; RRID:Addgene_125256)

  • For your References section:

    Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons. Osaki T, Uzel SGM, Kamm RD. Sci Adv. 2018 Oct 10;4(10):eaat5847. doi: 10.1126/sciadv.aat5847. eCollection 2018 Oct. 10.1126/sciadv.aat5847 PubMed 30324134
Related items:
Commonly requested with: