pGP-CMV-NES-fRCaMP2
(Plasmid #125250)

• Purpose: Expresses red fluorescent calcium sensor in mammalian cells
• Depositing Lab: Katalin Torok
• Publication: Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-3946. doi: 10.1074/jbc.RA118.004543. Epub 2019 Jan 16.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3955
• Total vector size (bp): 5341
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: fRCaMP2
• Alt name: jRCaMP1a EF-3
• Species: R. norvegicus (rat), G. gallus (chicken); E. quadricolor (Sea anemone)
• Insert Size (bp): 1386
• Mutation: D396A
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV
• 3′ sequencing primer: SV40p

Resource Information

• Supplemental Documents:
  • pGP-CMV-NES-fRCaMP2.txt
• A portion of this plasmid was derived from a plasmid made by: Addgene #61562

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

fRCaMP2 is the D396A variant of the jRCaMP1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.