pBabe puro HA PIK3CA E545K
(Plasmid #12525)

• Depositing Lab: Jean Zhao
• Publication: Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8.

Backbone

• Vector backbone: pBabe puro
• Backbone manufacturer: Available at Addgene (plasmid #1764)
• Backbone size w/o insert (bp): 5150
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PIK3CA E545K
• Alt name: phosphoinositide 3-kinase
• Alt name: PI3K
• Alt name: p110 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 3277
• Mutation: E545K. Tumor-derived allele.
• Entrez Gene: PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 18 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutation generated using Strategene Quickchange site-directed mutagenesis kit. See article for primer information.

Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val (I143V when compared to NP_006209.2). It could be a SNP. No functional changes have been observed so far in signaling assays.