pGP-CMV-NES-fRGECO1
(Plasmid #125247)

• Purpose: Expresses red fluorescent calcium sensor in mammalian cells
• Depositing Lab: Katalin Torok
• Publication: Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-3946. doi: 10.1074/jbc.RA118.004543. Epub 2019 Jan 16.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3955
• Total vector size (bp): 5368
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: fRGECO2
• Alt name: jRGECO1a RS-1 EF-4
• Species: R. norvegicus (rat), G. gallus (chicken); Discosoma sp. (Sea anemone)
• Insert Size (bp): 1413
• Tag / Fusion Protein:
  • NES signal

Resource Information

• Supplemental Documents:
  • pGP-CMV-NES-fRGECO1.txt
• A portion of this plasmid was derived from a plasmid made by: Addgene #61563

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

fRGECO1 is the D323A variant of the jRGECO1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.