plenti-ATF4
(Plasmid #125238)

• Purpose: ATF4 expression vector
• Depositing Lab: Rozita Yarmand
• Publication: Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61. doi: 10.1074/jbc.M114.619833. Epub 2015 Mar 20.

Backbone

• Vector backbone: pLenti-c-Myc-DDK-IRES-Puro
• Backbone manufacturer: origene
• Backbone size w/o insert (bp): 7600
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ATF4
• Species: H. sapiens (human)
• Entrez Gene: ATF4 (a.k.a. CREB-2, CREB2, TAXREB67, TXREB)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry