pBabe puro Myr HA PIK3CA
(Plasmid #12523)

• Depositing Lab: Jean Zhao
• Publication: Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8.

Backbone

• Vector backbone: pBabe puro
• Backbone manufacturer: Available at Addgene (plasmid #1764)
• Backbone size w/o insert (bp): 5150
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PIK3CA Myr
• Alt name: phosphoinositide 3-kinase
• Alt name: PI3K
• Alt name: p110 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 3300
• Mutation: Myristoylation. Constitutively active allele; I143V
• Entrez Gene: PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
• Tags / Fusion Proteins:
  • Myr (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make an amino-terminal myristoylated allele of p110, a coding sequence for the myristoylation domain of c-Src was fused in-frame with a HA-tagged PIK3CA.