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pUAST-attB-3xHA-V5-RpL10Ab
(Plasmid #125223)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASTattB
  • Backbone manufacturer
    Konrad Basler
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RpL10Ab
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1516
  • Entrez Gene
    RpL10Ab (a.k.a. Dmel_CG7283, BcDNA:RH06366, CG7283, Dmel\CG7283, L10Ab, RpL10A, RpL10Ae, anon-EST:Posey188)
  • Tag / Fusion Protein
    • 3x HA, V5 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the backbone for this plasmid is pUAST and not pUASTattB. This does not affect plasmid function as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-attB-3xHA-V5-RpL10Ab was a gift from Herman Dierick (Addgene plasmid # 125223 ; http://n2t.net/addgene:125223 ; RRID:Addgene_125223)

  • For your References section:

    A versatile method for cell-specific profiling of translated mRNAs in Drosophila. Thomas A, Lee PJ, Dalton JE, Nomie KJ, Stoica L, Costa-Mattioli M, Chang P, Nuzhdin S, Arbeitman MN, Dierick HA. PLoS One. 2012;7(7):e40276. doi: 10.1371/journal.pone.0040276. Epub 2012 Jul 6. 10.1371/journal.pone.0040276 PubMed 22792260
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