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Addgene

pBS_KS_attB2_SA(1)_T2AGeneSwitch
(Plasmid #125215)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS-KS-attB-1-2-PT-SA-SD-1
  • Backbone manufacturer
    Koen Venken
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GeneSwitch
  • Insert Size (bp)
    2875
  • Tag / Fusion Protein
    • T2A (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS_KS_attB2_SA(1)_T2AGeneSwitch was a gift from Herman Dierick (Addgene plasmid # 125215 ; http://n2t.net/addgene:125215 ; RRID:Addgene_125215)

  • For your References section:

    Gene-specific cell labeling using MiMIC transposons. Gnerer JP, Venken KJ, Dierick HA. Nucleic Acids Res. 2015 Apr 30;43(8):e56. doi: 10.1093/nar/gkv113. Epub 2015 Feb 20. 10.1093/nar/gkv113 PubMed 25712101
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