pBS_KS_attB2_SA(2)_T2AGAL4
(Plasmid
#125213)
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PurposeArtificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125213 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS-KS-attB-1-2-PT-SA-SD-2
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Backbone manufacturerKoen Venken
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGAL4
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3215
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Tag
/ Fusion Protein
- T2A (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS_KS_attB2_SA(2)_T2AGAL4 was a gift from Herman Dierick (Addgene plasmid # 125213 ; http://n2t.net/addgene:125213 ; RRID:Addgene_125213) -
For your References section:
Gene-specific cell labeling using MiMIC transposons. Gnerer JP, Venken KJ, Dierick HA. Nucleic Acids Res. 2015 Apr 30;43(8):e56. doi: 10.1093/nar/gkv113. Epub 2015 Feb 20. 10.1093/nar/gkv113 PubMed 25712101
