pAd-RFP
(Plasmid #12520)

• Depositing Lab: Tong-Chuan He
• Publication: Unpublished He lab plasmids (unpublished)

Backbone

• Vector backbone: pAdTrace-TO4
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Roger Tsien, UCSD
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is an RFP expressing adenoviral vector compatible with the AdEasy system. The mRFP is functional and the depositing lab has been using it as a control. The stop codon was originally deleted in order to make c-terminal fusion proteins. But the current construct should run into certain stop codons at the end of the linker sites.