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Addgene

TEVSH
(Plasmid #125194)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125194 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTH24 based on pDEST42
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 6536
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TEV_SH
  • Species
    Tobacco etch virus
  • Insert Size (bp)
    888
  • Promoter T7lac
  • Tag / Fusion Protein
    • V5 His-tag (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may run a dimer (>13kb) or multimer.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TEVSH was a gift from Helena Berglund (Addgene plasmid # 125194 ; http://n2t.net/addgene:125194 ; RRID:Addgene_125194)

  • For your References section:

    Improved solubility of TEV protease by directed evolution. van den Berg S, Lofdahl PA, Hard T, Berglund H. J Biotechnol. 2006 Feb 10;121(3):291-8. doi: 10.1016/j.jbiotec.2005.08.006. Epub 2005 Sep 15. 10.1016/j.jbiotec.2005.08.006 PubMed 16150509
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