pVP2del50
(Plasmid
#125185)
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PurposeExpresses the VP2del50 truncation mutant protein derived from AAV2 VP2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXX2
- Backbone size w/o insert (bp) 8000
- Total vector size (bp) 8000
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Modifications to backboneKnocked out all cap gene start codons for VP1, VP2 and VP3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehexahistidine tag
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Insert Size (bp)18
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MutationInserted start codon and hexahistidine tag at 2697
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GCG TAT CAG AAA CTG TGC TAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVP2del50 was a gift from Junghae Suh (Addgene plasmid # 125185 ; http://n2t.net/addgene:125185 ; RRID:Addgene_125185) -
For your References section:
Reprogramming the Activatable Peptide Display Function of Adeno-Associated Virus Nanoparticles. Thadani NN, Dempsey C, Zhao J, Vasquez SM, Suh J. ACS Nano. 2018 Feb 27;12(2):1445-1454. doi: 10.1021/acsnano.7b07804. Epub 2017 Dec 26. 10.1021/acsnano.7b07804 PubMed 29278489