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Addgene

pcDNA-mTRAF3ip3-CFP
(Plasmid #125181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-CFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRAF3ip3
  • Species
    M. musculus (mouse)
  • Mutation
    K87R, M283I, S359P, A506G- please see depositor comments below
  • Entrez Gene
    Traf3ip3 (a.k.a. 6030423D04Rik, AI115021, T3jam)
  • Promoter CMV
  • Tag / Fusion Protein
    • CFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms these mutations from NCBI reference NP_694777.3 do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-mTRAF3ip3-CFP was a gift from Shao-Cong Sun (Addgene plasmid # 125181 ; http://n2t.net/addgene:125181 ; RRID:Addgene_125181)
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