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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAdTrack-CMV
- Backbone size w/o insert (bp) 9220
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Vector typeMammalian Expression, Adenoviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWnt3A
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SpeciesM. musculus (mouse)
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Entrez GeneWnt3a (a.k.a. Wnt-3a, vt)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRoel Nusse, Stanford
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAd-Wnt3a was a gift from Tong-Chuan He (Addgene plasmid # 12518 ; http://n2t.net/addgene:12518 ; RRID:Addgene_12518) -
For your References section:
Connective tissue growth factor (CTGF) is regulated by Wnt and bone morphogenetic proteins signaling in osteoblast differentiation of mesenchymal stem cells. Luo Q, Kang Q, Si W, Jiang W, Park JK, Peng Y, Li X, Luu HH, Luo J, Montag AG, Haydon RC, He TC. J Biol Chem. 2004 Dec 31. 279(53):55958-68. 10.1074/jbc.M407810200 PubMed 15496414