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Addgene

pCLXSN(GFP)-HA-Tbkbp1 1-280
(Plasmid #125178)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125178 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCLXSN(GFP)-HA
  • Backbone manufacturer
    Obtained from Dr. Inder Verma and modified in Dr. Shao-Cong's lab. Addgene Plasmid #27557
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tbkbp1
  • Species
    M. musculus (mouse)
  • Mutation
    Contains amino acids 1-280
  • Entrez Gene
    Tbkbp1 (a.k.a. 3110043L15Rik, ProSAPiP2, SINTBAD)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pLXSN5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLXSN(GFP)-HA-Tbkbp1 1-280 was a gift from Shao-Cong Sun (Addgene plasmid # 125178 ; http://n2t.net/addgene:125178 ; RRID:Addgene_125178)

  • For your References section:

    TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, Shi J, Zhao S, Zhang B, Cheng X, Sun SC. Nat Commun. 2018 Jul 18;9(1):2812. doi: 10.1038/s41467-018-05097-5. 10.1038/s41467-018-05097-5 PubMed 30022064
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