Skip to main content
Addgene

pFB1.HMBP.PrS.PHF19
(Plasmid #125168)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125168 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFB1.HMBP.A3.PrS.ybbR (parent pFast.Bac1)
  • Backbone size w/o insert (bp) 5986
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PHF19
  • Alt name
    PHD finger protein 19
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1770
  • GenBank ID
    26147 26147
  • Entrez Gene
    PHF19 (a.k.a. MTF2L1, PCL3, TDRD19B)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • His-MBP (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer U003F pFB1 GGATTATTCATACCGTCCCA
  • 3′ sequencing primer U004 [Univ. pFB1/pFBD[PH] R] CAAATGTGGTATGGCTGATT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB1.HMBP.PrS.PHF19 was a gift from Chen Davidovich (Addgene plasmid # 125168 ; http://n2t.net/addgene:125168 ; RRID:Addgene_125168)

  • For your References section:

    RNA exploits an exposed regulatory site to inhibit the enzymatic activity of PRC2. Zhang Q, McKenzie NJ, Warneford-Thomson R, Gail EH, Flanigan SF, Owen BM, Lauman R, Levina V, Garcia BA, Schittenhelm RB, Bonasio R, Davidovich C. Nat Struct Mol Biol. 2019 Mar;26(3):237-247. doi: 10.1038/s41594-019-0197-y. Epub 2019 Mar 4. 10.1038/s41594-019-0197-y PubMed 30833789
Related items:
Commonly requested with: