pGL3_4xUAS_CP-candidate_luc+
(Plasmid
#125154)
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PurposeSTAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneUnknown
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4779
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4 x upstream activating sequence (UAS)
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)177
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3_4xUAS_CP-candidate_luc+ was a gift from Alexander Stark (Addgene plasmid # 125154 ; http://n2t.net/addgene:125154 ; RRID:Addgene_125154) -
For your References section:
Transcriptional cofactors display specificity for distinct types of core promoters. Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A. Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. Epub 2019 May 15. 10.1038/s41586-019-1210-7 PubMed 31092928
