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pSTAP-seq_human-4xUAS
(Plasmid #125150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2500
  • Vector type
    STAP-seq screening vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4 x upstream activating sequence (UAS)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    181

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSTAP-seq_human-4xUAS was a gift from Alexander Stark (Addgene plasmid # 125150 ; http://n2t.net/addgene:125150 ; RRID:Addgene_125150)

  • For your References section:

    Transcriptional cofactors display specificity for distinct types of core promoters. Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A. Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. Epub 2019 May 15. 10.1038/s41586-019-1210-7 PubMed 31092928
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