pSTAP-seq_fly-4xUAS
(Plasmid
#125149)
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PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3078
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Vector typeSTAP-seq screening vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4 x upstream activating sequence (UAS)
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)187
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSTAP-seq_fly-4xUAS was a gift from Alexander Stark (Addgene plasmid # 125149 ; http://n2t.net/addgene:125149 ; RRID:Addgene_125149) -
For your References section:
Transcriptional cofactors display specificity for distinct types of core promoters. Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A. Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. Epub 2019 May 15. 10.1038/s41586-019-1210-7 PubMed 31092928