pMOLUC
(Plasmid
#12514)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonena
- Backbone size (bp) 3228
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Vector typeBacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (unknown if destroyed)
- 3′ cloning site See map (unknown if destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMOLUC was a gift from Tong-Chuan He (Addgene plasmid # 12514 ; http://n2t.net/addgene:12514 ; RRID:Addgene_12514) -
For your References section:
Increased efficiency of cloning large DNA fragments using a lower copy number plasmid. Feng T, Li Z, Jiang W, Breyer B, Zhou L, Cheng H, Haydon RC, Ishikawa A, Joudeh MA, He TC. Biotechniques. 2002 May . 32(5):992, 994, 996 passim. 10.2144/02325bm03 PubMed 12019795
Map uploaded by the depositor.
