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Addgene

Double UP mScarlet to mNeon
(Plasmid #125139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCax
  • Backbone size (bp) 6734
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    Removed Not1 site in mScarlet

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mScarlet can act as an internal control to mNeon for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mNeon through use of a mcs after the P2A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Double UP mScarlet to mNeon was a gift from Erik Dent (Addgene plasmid # 125139 ; http://n2t.net/addgene:125139 ; RRID:Addgene_125139)

  • For your References section:

    Double UP: A Dual Color, Internally Controlled Platform for in utero Knockdown or Overexpression. Taylor RJ, Carrington J, Gerlach LR, Taylor KL, Richters KE, Dent EW. Front Mol Neurosci. 2020 May 20;13:82. doi: 10.3389/fnmol.2020.00082. eCollection 2020. 10.3389/fnmol.2020.00082 PubMed 32508591
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