Double UP Halotag to mScarlet
(Plasmid #125138)

• Purpose: (Empty Backbone) Natively produces Halotag, in the presence of Cre will be recombined to produce mScarlet.
• Depositing Lab: Erik Dent
• Publication: Taylor et al Front Mol Neurosci. 2020 May 20;13:82. doi: 10.3389/fnmol.2020.00082. eCollection 2020.

Backbone

• Vector backbone: pCAX
• Backbone size (bp): 6926
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Mutation: Removed Not1 site in mScarlet

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that Halotag can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.

Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.