Double UP mNeon to mScarlet P2A Rac1-wt
(Plasmid
#125135)
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PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged WT Rac1.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAX
- Backbone size w/o insert (bp) 6734
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeon/mScarletP2A-Rac1
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Alt nameRac1-WT
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Alt name5879
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MutationRemoved Not1 site in mScarlet
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CAGCTCCTGGGCAACGTGC
- 3′ sequencing primer m13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. WT-Rac1 is expressed following a P2A site, so that Cre+ cells will be both mScarlet positive and also contain an untagged WT Rac1.
Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Double UP mNeon to mScarlet P2A Rac1-wt was a gift from Erik Dent (Addgene plasmid # 125135 ; http://n2t.net/addgene:125135 ; RRID:Addgene_125135) -
For your References section:
Double UP: A Dual Color, Internally Controlled Platform for in utero Knockdown or Overexpression. Taylor RJ, Carrington J, Gerlach LR, Taylor KL, Richters KE, Dent EW. Front Mol Neurosci. 2020 May 20;13:82. doi: 10.3389/fnmol.2020.00082. eCollection 2020. 10.3389/fnmol.2020.00082 PubMed 32508591