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pCAGGS-Akt-FoxO3a-KTR-EGFP
(Plasmid #125131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone manufacturer
    Junichi Miyazaki (Osaka University, Japan)
  • Backbone size w/o insert (bp) 4870
  • Total vector size (bp) 8551
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOXO2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1206
  • Mutation
    FoxO3A 1-402 amino acid
  • GenBank ID
    NM_001455
  • Entrez Gene
    FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
  • Promoter CAG promoter
  • Tag / Fusion Protein
    • Enhanced green fluorescent protein (EGFP) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAGGSseq_F
  • 3′ sequencing primer pCAGGSseq_R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression vector for Akt-FoxO3a-KTR-EGFP in mammalian cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-Akt-FoxO3a-KTR-EGFP was a gift from Kazuhiro Aoki (Addgene plasmid # 125131 ; http://n2t.net/addgene:125131 ; RRID:Addgene_125131)

  • For your References section:

    Multiplexed Fluorescence Imaging of ERK and Akt Activities and Cell-cycle Progression. Maryu G, Matsuda M, Aoki K. Cell Struct Funct. 2016 Jul 22;41(2):81-92. doi: 10.1247/csf.16007. Epub 2016 May 28. 10.1247/csf.16007 PubMed 27247077
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