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Addgene

pTLOX
(Plasmid #12513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    na
  • Backbone size (bp) 8390
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (unknown if destroyed)
  • 3′ cloning site See map (unknown if destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A LoxP-flanked
stuffer containing GFP and the SV40 PA signal was cloned into the pTHE vector before the multiple cloning
site and after the TetR-responsive
elements (TO4).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTLOX was a gift from Tong-Chuan He (Addgene plasmid # 12513 ; http://n2t.net/addgene:12513 ; RRID:Addgene_12513)

  • For your References section:

    Dual regulation of gene expression mediated by tetracycline and Cre recombinase. Park JY, Luo Q, Jiang W, Kang Q, Peng Y, Strom C, Luu HH, Haydon RC, He TC. Biotechniques. 2004 Mar . 36(3):390-2, 394, 396. 10.2144/04363BM03 PubMed 15038152
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