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Addgene

pMinDis.X513-iNicSnFR3a-(CC93)-ER
(Plasmid #125121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMinDis.X513
  • Backbone manufacturer
    Janelia
  • Backbone size w/o insert (bp) 5005
  • Total vector size (bp) 6655
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) for bacterial expression and grow at 30 degrees C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iNicSnFR3a-(CC93)-ER
  • Alt name
    iNicSnFR3a-(CC93)-ER
  • Species
    Mammilian
  • Insert Size (bp)
    1650
  • Promoter T7
  • Tag / Fusion Protein
    • ER retention motif (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site pstI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that a 6bp insertion and mutation V183A was identified in the NeoR/KanR cassette during Addgene's quality control process. We recommend testing NeoR selection before performing experiments. Vector was received from Janelia HHMI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMinDis.X513-iNicSnFR3a-(CC93)-ER was a gift from Henry Lester (Addgene plasmid # 125121 ; http://n2t.net/addgene:125121 ; RRID:Addgene_125121)

  • For your References section:

    Determining the pharmacokinetics of nicotinic drugs in the endoplasmic reticulum using biosensors. Shivange AV, Borden PM, Muthusamy AK, Nichols AL, Bera K, Bao H, Bishara I, Jeon J, Mulcahy MJ, Cohen B, O'Riordan SL, Kim C, Dougherty DA, Chapman ER, Marvin JS, Looger LL, Lester HA. J Gen Physiol. 2019 Feb 4. pii: jgp.201812201. doi: 10.1085/jgp.201812201. 10.1085/jgp.201812201 PubMed 30718376
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