pTHE
(Plasmid #12512)

• Purpose: (Empty Backbone)
• Depositing Lab: Tong-Chuan He
• Publication: Jiang et al J Biol Chem. 2001 Nov 30. 276(48):45168-74.

Backbone

• Vector backbone: pCMV-TO4
• Backbone size (bp): 7218
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (unknown if destroyed)
• 3′ cloning site: See map (unknown if destroyed)
• 5′ sequencing primer: na

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCMV-TO4 has four copies of the tet operator site tetO2 and multiple cloning sites cloned downstream to a CMV promoter. An MluI fragment containing the TRSID expression cassette (CMV-TRSID-IRES-Neo-SV40 PA) was subcloned into the pCMV-TO4 vector.