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Addgene

pSR322.3
(Plasmid #125118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProTet.E333
  • Total vector size (bp) 4651
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW29852
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    uhpA(REC)-ydfI(DBD)129
  • Species
    Synthetic
  • Insert Size (bp)
    633

Gene/Insert 2

  • Gene/Insert name
    sfgfp
  • Alt name
    Super folder GFP
  • Species
    Synthetic
  • Insert Size (bp)
    714

Gene/Insert 3

  • Gene/Insert name
    tetR
  • Species
    Tn10 from E. coli
  • Insert Size (bp)
    625
  • GenBank ID
    NC_009133.1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSR322.3 was a gift from Jeffrey Tabor (Addgene plasmid # 125118 ; http://n2t.net/addgene:125118 ; RRID:Addgene_125118)

  • For your References section:

    Rewiring bacterial two-component systems by modular DNA-binding domain swapping. Schmidl SR, Ekness F, Sofjan K, Daeffler KN, Brink KR, Landry BP, Gerhardt KP, Dyulgyarov N, Sheth RU, Tabor JJ. Nat Chem Biol. 2019 Jul;15(7):690-698. doi: 10.1038/s41589-019-0286-6. Epub 2019 May 20. 10.1038/s41589-019-0286-6 PubMed 31110305
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