pKD279
(Plasmid
#125099)
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PurposeExpression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepProTet.E333
- Total vector size (bp) 4715
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameSO_4388(REC)-psdR(DBD)137
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SpeciesSynthetic
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Insert Size (bp)702
Gene/Insert 2
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Gene/Insert namesfgfp
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Alt nameSuper folder GFP
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SpeciesSynthetic
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Insert Size (bp)714
Gene/Insert 3
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Gene/Insert nametetR
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SpeciesTn10 from E. coli
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Insert Size (bp)625
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GenBank IDNC_009133.1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKD279 was a gift from Jeffrey Tabor (Addgene plasmid # 125099 ; http://n2t.net/addgene:125099 ; RRID:Addgene_125099) -
For your References section:
Rewiring bacterial two-component systems by modular DNA-binding domain swapping. Schmidl SR, Ekness F, Sofjan K, Daeffler KN, Brink KR, Landry BP, Gerhardt KP, Dyulgyarov N, Sheth RU, Tabor JJ. Nat Chem Biol. 2019 Jul;15(7):690-698. doi: 10.1038/s41589-019-0286-6. Epub 2019 May 20. 10.1038/s41589-019-0286-6 PubMed 31110305