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Addgene

pKD280
(Plasmid #125071)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125071 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYCDuet-1
  • Total vector size (bp) 5083
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    SO_4387
  • Insert Size (bp)
    1500
  • Entrez Gene
    SO_4387 (a.k.a. SO_4387)

Gene/Insert 2

  • Gene/Insert name
    lacI
  • Insert Size (bp)
    957
  • Mutation
    S286L- Please see depositor comments below
  • Entrez Gene
    lacI (a.k.a. b0345, ECK0342)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms mutation does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKD280 was a gift from Jeffrey Tabor (Addgene plasmid # 125071 ; http://n2t.net/addgene:125071 ; RRID:Addgene_125071)

  • For your References section:

    Rewiring bacterial two-component systems by modular DNA-binding domain swapping. Schmidl SR, Ekness F, Sofjan K, Daeffler KN, Brink KR, Landry BP, Gerhardt KP, Dyulgyarov N, Sheth RU, Tabor JJ. Nat Chem Biol. 2019 Jul;15(7):690-698. doi: 10.1038/s41589-019-0286-6. Epub 2019 May 20. 10.1038/s41589-019-0286-6 PubMed 31110305
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