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Addgene

pCMV-3xFlag-ETO
(Plasmid #12507)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12507 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFlag-CMV-7.1
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ETO
  • Species
    H. sapiens (human)
  • Entrez Gene
    RUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
  • Tag / Fusion Protein
    • 3xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (blunted) (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pCEP fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-3xFlag-ETO was a gift from Dong-Er Zhang (Addgene plasmid # 12507 ; http://n2t.net/addgene:12507 ; RRID:Addgene_12507)

  • For your References section:

    AML1/RUNX1 phosphorylation by cyclin-dependent kinases regulates the degradation of AML1/RUNX1 by the anaphase-promoting complex. Biggs JR, Peterson LF, Zhang Y, Kraft AS, Zhang DE. Mol Cell Biol. 2006 Oct . 26(20):7420-9. 10.1128/MCB.00597-06 PubMed 17015473
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