-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 12503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepIRES-EGFP
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5300
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSynaptotagmin IV
-
Alt nameSyt IV
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)1400
-
Entrez GeneSyt4
-
Tag
/ Fusion Protein
- IRES EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
rSytIV pIE was a gift from Jane Sullivan (Addgene plasmid # 12503 ; http://n2t.net/addgene:12503 ; RRID:Addgene_12503) -
For your References section:
Synaptotagmin IV does not alter excitatory fast synaptic transmission or fusion pore kinetics in mammalian CNS neurons. Ting JT, Kelley BG, Sullivan JM. J Neurosci. 2006 Jan 11. 26(2):372-80. 10.1523/JNEUROSCI.3997-05.2006 PubMed 16407532