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Addgene

pCW57.1-DUXB-CA
(Plasmid #125027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1
  • Backbone size w/o insert (bp) 9354
  • Total vector size (bp) 8819
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    We used Stbl3 cells (recombination deficient)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DUXB (codon altered)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1038
  • Mutation
    codon altered
  • GenBank ID
    NM_001351307
  • Entrez Gene
    DUXB
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer tcgcctggagaattggc
  • 3′ sequencing primer agggctgccttggaaaag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1-DUXB-CA was a gift from Stephen Tapscott (Addgene plasmid # 125027 ; http://n2t.net/addgene:125027 ; RRID:Addgene_125027)

  • For your References section:

    DUX4 Suppresses MHC Class I to Promote Cancer Immune Evasion and Resistance to Checkpoint Blockade. Chew GL, Campbell AE, De Neef E, Sutliff NA, Shadle SC, Tapscott SJ, Bradley RK. Dev Cell. 2019 Sep 9;50(5):658-671.e7. doi: 10.1016/j.devcel.2019.06.011. Epub 2019 Jul 18. 10.1016/j.devcel.2019.06.011 PubMed 31327741
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