p5E-0.349cldn15la
(Plasmid #125026)

• Purpose: Five prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.
• Depositing Lab: John Rawls
• Publication: Murdoch et al PLoS Pathog. 2019 Mar 7;15(3):e1007381. doi: 10.1371/journal.ppat.1007381. eCollection 2019 Mar.

Backbone

• Vector backbone: p5E-Fse-Asc 381
• Backbone size w/o insert (bp): 2663
• Total vector size (bp): 3012

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Claudin 15-like a
• Alt name: cldn15la
• Species: D. rerio (zebrafish)
• Insert Size (bp): 349
• Entrez Gene: cldn15la (a.k.a. cldn10, cldn10l, cldn10l1a)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • p5E-cldn15la.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry