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Addgene

6379 H10-K218T
(Plasmid #1250)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC45S
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp104 (K218T)
  • Alt name
    Hsp104
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    K218T
  • Entrez Gene
    HSP104 (a.k.a. YLL026W)
  • Tag / Fusion Protein
    • his tag (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    John Glover

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6379 H10-K218T was a gift from Susan Lindquist (Addgene plasmid # 1250 ; http://n2t.net/addgene:1250 ; RRID:Addgene_1250)

  • For your References section:

    Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins. Glover JR, Lindquist S. Cell 1998 Jul 10;94(1):73-82. 10.1016/S0092-8674(00)81223-4 PubMed 9674429
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