RV-mCD4-HH5-5
(Plasmid
#124985)
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PurposeExpress the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124985 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMigR1
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Vector typeMammalian Expression ; Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHH5-5 TCRa P2A TCRb
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SpeciesM. musculus (mouse)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RV-mCD4-HH5-5 was a gift from Dan Littman (Addgene plasmid # 124985 ; http://n2t.net/addgene:124985 ; RRID:Addgene_124985) -
For your References section:
c-MAF-dependent regulatory T cells mediate immunological tolerance to a gut pathobiont. Xu M, Pokrovskii M, Ding Y, Yi R, Au C, Harrison OJ, Galan C, Belkaid Y, Bonneau R, Littman DR. Nature. 2018 Feb 15;554(7692):373-377. doi: 10.1038/nature25500. Epub 2018 Feb 7. 10.1038/nature25500 PubMed 29414937