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Addgene

pD21-AI-dsRed
(Plasmid #124977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pD21
  • Backbone manufacturer
    BCM
  • Backbone size w/o insert (bp) 24000
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dsRed
  • Species
    Synthetic
  • Promoter human APOAI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site AscI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositors sequence. We recommend running additional diagnostic tests before use.

Depositor also recommends growing plasmid at 30 degrees C due to their size.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD21-AI-dsRed was a gift from Kazuhiro Oka (Addgene plasmid # 124977 ; http://n2t.net/addgene:124977 ; RRID:Addgene_124977)
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