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Addgene

pCDH-CMV-mC-G-LC3B-P
(Plasmid #124974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV (Addgene #72265)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-GFP-LC3B
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-CMV-mC-G-LC3B-P was a gift from Kazuhiro Oka (Addgene plasmid # 124974 ; http://n2t.net/addgene:124974 ; RRID:Addgene_124974)

  • For your References section:

    RAS-related GTPases DIRAS1 and DIRAS2 induce autophagic cancer cell death and are required for autophagy in murine ovarian cancer cells. Sutton MN, Yang H, Huang GY, Fu C, Pontikos M, Wang Y, Mao W, Pang L, Yang M, Liu J, Parker-Thornburg J, Lu Z, Bast RC Jr. Autophagy. 2018;14(4):637-653. doi: 10.1080/15548627.2018.1427022. Epub 2018 Mar 21. 10.1080/15548627.2018.1427022 PubMed 29368982
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